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13.1 13.2 13.3

(4.99) (4.100)

each cell type is then labelled with a different uorescent dye (e.g. green for control and red for test). The two populations are washed over the microarray and allowed to hybridize to the DNA printed on the spots. If a gene is preferentially expressed in the test sample, the spot will glow red when illuminated with a laser beam. If a gene is preferentially expressed in the control sample (due to down-regulation), the spot will glow green. If a gene is equally expressed in both test and control, the spot will glow yellow. Image analysis software provides readout of this experiment in terms of spot identi cation and quanti cation. Microarrays usually come with built-in blank spots, control spots and at least duplicate spots for each gene. By comparing known levels of genes on a number of microarrays with observed spot intensities for genes under investigation it is possible to generate a gene expression matrix for the cells under investigation which can be related to results from other experiments. 10.3.2 Protein Biochips

Therefore, it is clear that both CFD techniques are able to visualize the flow structure in this region However, it is worth noting that the LGD simulation is unsteady whereas the RANS solution has been run as a steady-state simulation Thus the added CPU cost (Table 31) of the LGD method is justified, at least in part, by the opportunity to examine any flow unsteadiness that may be present 3533 Flow close to the tail of the train ('near-wake') As stated at the outset, the primary focus for MIRA's involvement in RAPIDE and thus the case study developed from the project, was to model flow around the leading vehicle and first carriage of the train However, it was more convenient to use the LGD model to represent a consist including a rear-facing driving trailer (ie tail car).

Summary

Because of inertia effects, spring tides happen a couple of days after the time when the Sun, Moon and Earth are in line Spring tides occur, then, a couple of days after the New Moon or the Full Moon are seen Neap Tides When the line joining the Earth and Moon is at right angles to that joining the Earth and Sun, the gravitational force on the Earth and oceans is minimum and so the gravitational bulge is a minimum also The tidal range at this time is minimum and these lower tides are called neap tides Neap tides are associated with Half Moon , but inertia also affects this; so neap tides occur around 2 days after the rst and second Half Moon Tides change from spring tides to neap tides and back to spring tides over a 14-day period.

Therefore, the data set from the LGD CFD exercise is more comprehensive than that obtained using the RANS code This provides an added opportunity to assess the visualization capability of the LGD method by assessing how well it predicts the flow structure around the tail of the train This is an important feature of the flow, as this is the early part of the vehicle wake The wake of a train has a significant impact, along with the slipstream in general, on the security of people (both maintenance workers and passengers waiting at platforms) and objects at the trackside Thus, from an engineering perspective the ability to determine the flow structure in this 'near-wake' region is an important one At present, as the RAPIDE project is not yet complete2 data with which to correlate the LGD predictions are sparse, limited to the items listed below.

The heights of a spring and neap tides vary throughout the year, the highest springs being associated approximately with the equinoxes in the spring and autumn; hence the term spring tides The lowest neaps are associated approximately with the solstices, the origin of the term neap being unclear but probably originating from the old English nep od used to describe the lowest high waters Tidal Range Whether a tide is neap or spring (or an intermediate) is determined not by the height of high water but by the difference between the height of high water and the next (or preceding) low water This is called the tidal range Spring tides have a large range and neap tides a small one The mean sea level remains roughly constant.