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where is the vibrational frequency, k the classical force constant, and u = m1 m2 ( m1 + m2 ) , the reduced mass of the two atoms. The model provides a good description of true diatomic molecules and is not far from the average value of two atoms stretching within a polyatomic molecule. The corresponding potential-energy curve is the typical parabola illustrated in Figure 8. This approximation gives the average vibration frequency of the bond. For example, the reduced masses for C H, O H, and N H are 0.85, 0.89, and 0.87. These gures are similar, so the frequencies would be quite similar too. However, the electron-withdrawing and -donating properties of neighbors within molecules act

(9.38a) (9.38b) (9.38c)

Example 6.5 Determination of the Structure of a Binding Domain of the Human Editing Enzyme ADAR1 Bound to DNA Almost three decades after the structure of right-handed double helix B-DNA was elucidated by Watson and Crick, a separate left-handed form of DNA called Z-DNA was discovered which was of unknown biological function. Unlike the smooth helix traced by the phosphates of B-DNA which form relatively shallow major and minor grooves, the phosphates of Z-DNA trace a jagged helix which forms a single deep groove extending to the axis of the helix. An enzyme called RNA adenosine deaminase (ADAR1) was found to bind to Z-DNA quite strongly and speci cally by band-shift assays ( 5). This binding activity was localized in a speci c portion of the protein (the Z domain). Z-DNA is formed transiently in the wake of a moving RNA polymerase and is thought to be stabilized by negative supercoiling. ADAR1 is thought therefore to target actively transcribing genes. In order to understand this biological function in greater detail, an investigation of the detailed structural interactions between this protein and DNA was carried out using X-ray diffraction. Cubic crystals of the Z domain (residues 133 209 of ADAR1) and an oligonucleotide of sequence (TCGCGCG)2 were obtained with ammonium sulfate as precipitant by vapour diffusion using the hanging drop method. The cell dimensions were a = b = 85.9 A, c = 71.3 A. Single isomorphous crystals were obtained by including 5-iodouracil for thymine-1 (iodo-1) and 5-iodocytosine for 6-iodocytosine (iodo-6). Data was collected on a rotating anode X-ray source and the data collection details are summarized in (1) below. (1) Native Resolution range (A) Number of unique re ections Completeness 40 2.4 10 896 99.2 Iodo-1 20 2.1 29 702 99.9 Iodo-6 20 2.8 12 566 99.9

(4.93)

o drawing project is ever completed without changes. You make changes in a drawing for many reasons. Some editing processes are simply part of the drawing process, such as copying an object instead of drawing it a second time from scratch. Other types of editing involve making changes to many objects at once, such as moving an entire section of a drawing to make room for additional objects. You often also need to erase, move, rotate, and resize objects.

The circular and one-level representation are popular full representations that are complete The minimum suf cient representation is the minimal representation with no loss of information In the MSR adjacency graph given in Figure 191, R0,0 , R3,3 , and R3,0 are 1 since all the vertices, regions, and adjacency {M 3 {M 0 }} are present in the representation R1,1 and R2,2 are since only edges classi ed on model edges and faces classi ed on model faces are present R1,0 and R2,0 are since the downward adjacencies {M 1 {M 0 }} and {M 2 {M 0 }} / are stored only for the represented edges and faces The remaining Ri,j , i = j, are 0..

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The issubclass(class,class) checks to see if one class is a descendent of another:

Finally, displayCart() displays the user s cart, as well as the list of available products. After displaying an XHTML page header, the function loops through each item in the cart, displaying the product name, price, and a Remove link that allows the user to remove the product from his cart. It also totals the prices of all the products in the cart as it goes, then displays the total below the cart:

Although link creation doesn t have to be your last step, logically and in practice, it is likely to be. Before you do this, you at least have to have your content sources defined.

the bridge sees a source MAC address that it does not currently have in its address table, it will add the information at that point.

Most of the testing must be automated via commercial packages or scripts. These tests will be necessary to verify setup during implementation.